Use of the enzyme-linked immunosorbent assay technique (elisa) for the diagnosis of typhoid fever. I. Standardization of technique.

R, Hernández-Velarde; J, Sánchez-Castillo; M, Cielo Díaz-Godínez; O, Muñóz-Hernández

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Article . 1980

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Use of the enzyme-linked immunosorbent assay technique (elisa) for the diagnosis of typhoid fever. I. Standardization of technique.

descriptionPublicationkeyboard_double_arrow_right Article 23 Sep 1980Journal:Archivos de investigacion medica, volume 11 (issn: 0066-6769,

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Authors: R, Hernández-Velarde; J, Sánchez-Castillo; M, Cielo Díaz-Godínez; O, Muñóz-Hernández;

pmid: 6994674

Use of the enzyme-linked immunosorbent assay technique (elisa) for the diagnosis of typhoid fever. I. Standardization of technique.

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Abstract

The sera of 25 patients with bacteriological diagnosis of typhoid fever were analyzed to identify and measure the antibodies to S. typhi somatic antigens using the enzyme-linked immunosorbent assay technique (ELISA). The results were positive in all the serologic tests, with titers varying from 1:600 to 1:2500. Development and standardization of the technique, results of the intra-assay and inter-assay tests and their correlation to Widal's reaction are described in detail.

Keywords

Immunoenzyme Techniques, Adolescent, Humans, Enzyme-Linked Immunosorbent Assay, Typhoid Fever, Antibodies, Bacterial

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