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[Method of isolation of intact enterocytes from rat duodenal mucosa].

descriptionPublicationkeyboard_double_arrow_right Article 17 Jun 1983Journal:Ukrainskii biokhimicheskii zhurnal (1978), volume 55 (issn: 0201-8470, Copyright policy )
Authors: B F, Sukhomlinov; A N, Fedorovich; Ia P, Chaĭka;

pmid: 6845446

[Method of isolation of intact enterocytes from rat duodenal mucosa].

Abstract

A procedure is suggested for obtaining intact enterocytes from rat duodenum mucosa by the tissue trypsinization in the isotonic Krebs solution (pH 7.9). The maximum yield of certain enterocytes is reached at the enzyme concentration of 0.03%; the incubation time--15 min; 200-250 mg of mucosa produces 3,7 X 10(6)--4,7 X 10(6) cells.

Keywords

Male, Microscopy, Electron, Cell Survival, Duodenum, Animals, Cell Separation, Intestinal Mucosa, Rats

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
1
Average
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