The investigations were carried out on the material taken from four pigs, weight about 35 kg. Ovary ligaments and hilus were taken for these experiments. To trace cholinergic nerves situated in the collected material, Koelle and Friedenwald's thiocholine method in Gomori's modification and Gienc's adaptation was used. To eliminate the possibility of pseudocholinesterase staining in the preparation, Iso-OMPE in dilution 1 X 10(-6) was added to the control material. In the mesovarium of the pig cholinergic nerves have been observed. They can be divided into three groups according to their course and position: 1) paravascular bundles, 2) perivascular plexi and 3) nerve bundles not connected with the vessels.