Preparation of [14C]uridine 5'-diphosphate and [14C]guanosine 5'-diphosphate.

Name: Preparation of [14C]uridine 5'-diphosphate and [14C]guanosine 5'-diphosphate.
Keywords: Ribonucleoside Diphosphate Reductase, Uracil Nucleotides, Isotope Labeling, Animals, Cattle, Carbon Radioisotopes, Guanosine Diphosphate, Guanine Nucleotides, Uridine Diphosphate

J G, Cory; P E, Bacon

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Preparative Biochemi...arrow_drop_down

Preparative Biochemistry

Article . 1984

Data sources: Europe PubMed Central

Preparation of [14C]uridine 5'-diphosphate and [14C]guanosine 5'-diphosphate.

descriptionPublicationkeyboard_double_arrow_right Article 21 Nov 1984 English Journal:Preparative biochemistry, volume 14 (issn: 0032-7484,

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Authors: J G, Cory; P E, Bacon;

pmid: 6483806

Preparation of [14C]uridine 5'-diphosphate and [14C]guanosine 5'-diphosphate.

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Abstract

Procedures have been developed for the routine enzymatic synthesis of [14C]UDP and [14C]GDP from commercially available enzymes and [14C]UMP and [14C]GMP. Using high pressure liquid chromatography, the products are recovered in high yield (60-80%) and with high purity. The [14C]UDP and [14C]GDP are utilized as substrates for ribonucleotide reductase.

Keywords

Ribonucleoside Diphosphate Reductase, Uracil Nucleotides, Isotope Labeling, Animals, Cattle, Carbon Radioisotopes, Guanosine Diphosphate, Guanine Nucleotides, Uridine Diphosphate

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