Technique and results of 50 laparoscopies performed on sterility patients to obtain mature oocytes from Graafian follicles to carry out in vitro fertilisation, are reported. In 32% of all laparoscopies and in 52% of all punctured follicles a mature oocyte could be obtained. Different diameters of puncture needles as well as different suction intensities are compared. It is mentioned that through increasing experience and improved technique the exact time for the intervention has been correctly determined in 92%. The recovery-rate for oocytes also could be increased to 58%. A further improvement in this important initial step to a successful in vitro fertilisation program is feasible.