One-step Plasmodium falciparum cultivation--application to in-vitro drug testing.

P, Druilhe; D, Mazier; O, Brandicourt; M, Gentilini

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Article . 1984

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One-step Plasmodium falciparum cultivation--application to in-vitro drug testing.

descriptionPublicationkeyboard_double_arrow_right Article 14 Mar 1984 English Journal:Tropenmedizin und Parasitologie, volume 34 (issn: 0303-4208,

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Authors: P, Druilhe; D, Mazier; O, Brandicourt; M, Gentilini;

pmid: 6364513

One-step Plasmodium falciparum cultivation--application to in-vitro drug testing.

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Abstract

P. falciparum was cultivated by a one-step method which requires medium renewal and addition of fresh RBC only every three days and allows high proliferation rates of the parasite. The method has been used for routine maintenance of strains and applied to the in-vitro evaluation of the effect of antimalarial compounds over 3 days periods as measured by 3H-Hypoxanthine incorporation.

Keywords

Antimalarials, Erythrocytes, Plasmodium falciparum, Animals, Drug Evaluation, Humans, Parasitology, Culture Media

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