
The potential of the cloned histone H5 gene to form altered DNA structures has been examined by S1 nuclease digestion of supercoiled recombinant plasmids containing up to 8.8 kbp of chicken DNa. The three main nicking sites map at the upstream and downstream sequences flanking the structural gene. The cleavage sites share sequence homology, strand specificity, and do not seem to be single-stranded. The sequence of the S1-sensitive sites does not suggest that the fragments can adopt any of the known DNA secondary structures.
Base Sequence, DNA, Superhelical, Single-Strand Specific DNA and RNA Endonucleases, DNA, Recombinant, Nucleic Acid Hybridization, DNA Restriction Enzymes, Endonucleases, Histones, Genes, Animals, Cloning, Molecular, Chickens, Plasmids
Base Sequence, DNA, Superhelical, Single-Strand Specific DNA and RNA Endonucleases, DNA, Recombinant, Nucleic Acid Hybridization, DNA Restriction Enzymes, Endonucleases, Histones, Genes, Animals, Cloning, Molecular, Chickens, Plasmids
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| impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network. | Top 10% |
