The intermediate host of Dipetalonema witei, the soft tick Ornithodoros moubata, can be infected experimentally with microfilariae. Microfilariae isolated from the blood of infected donor animals were injected into the dorsal haemocoel of the ticks where they developed to infectious filariae within approx. 50 days. The rate of infection of ticks was 100% when more than 100 microfilariae were injected. An average of 23% was recovered as infectious filariae. Up to 450 ticks can be infected with 1000 microfilariae each from a single donor animal within one year, whereas donor animals can only be used for about 1 month if ticks are naturally infected. The method described has advantages compared to natural tick infections with respect to the rate of infection of ticks, the development of microfilariae, and the amount of infectious material produced.