
In Loyez-Woelcke's myelin sheath staining the author studied the effect of the tanning time, that of the ripening grade of hematoxylin as well as the effect of the staining time. In case of a determined staining time a certain proportion must be present between the ripening grade (or the quantity of the oxidizing agent added to the solution to enhance ripening) and the quantity of the alkalizing agent. On the other hand the absolute quantity of the two substances has only little influence on staining. A neutral red staining was found efficacious to demonstrate cell nuclei and Nissl substance.
Cell Nucleus, Time Factors, Staining and Labeling, Nissl Bodies, Methods, Humans, Myelin Sheath
Cell Nucleus, Time Factors, Staining and Labeling, Nissl Bodies, Methods, Humans, Myelin Sheath
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