The contraction of granulation tissue is an important factor in wound healing. The tract of an abdominal drainage tube closes at the rate of 1 mm/h once the tube is removed. Specialized myofibroblasts are generally believed to be responsible for this process and the presence of elastin in granulation tissue has been debated for many years. A 3-mm polyethylene tube was inserted through the left lobe of the liver in 41 rats. A tube of granulation tissue formed around the plastic. This granulation tissue was isolated, after periods ranging from 8 to 20 days, by removing the left lobe of the liver and scraping away the surrounding liver tissue. Biochemical assay for elastin revealed that normal hepatic tissue does not contain any detectable amount of elastin. All 41 samples of granulation tissue contained elastin with a mean value of 8.5 X 10(-6) g of elastin per milligram of tissue. Normal rat aorta contains 31.4 X 10(-6) g of elastin per milligram of tissue. These tubes of granulation tissue were histologically normal and the presence of elastin was recognized using elastin stains. The exact role of elastin in granulation tissue is uncertain but this experiment clearly demonstrates its generation and presence there.