The role of wild avian species in the natural history of influenza is unknown. A serological study was carried out to ascertain the prevalence, distribution, and types of influenza antibody in several wild Canada goose populations. Geese were trapped and blood samples were obtained in each of 4 consecutive years, 1966-69. Antibody to influenzavirus was found in 66 (4.7%) of the 1 401 Canada geese tested by the haemagglutination inhibition (HI) test. Antiribonucleoprotein antibody was found in 8 of 1 359 sera tested by the agar gel precipitation (AGP) test. An increase in the percentage of reactors was seen each year. This increase was greater in two refuges with nonmigratory flocks. HI antibody was found against the turkey/Wisconsin/66, turkey/Wisconsin/68, turkey/Canada/63, and turkey/Alberta/6962/66, or closely related viruses. No antibody was found against duck/Ukraine/1/63 or human A/Hong Kong/68 virus at a time when the latter was prevalent in human populations, suggesting that Canada geese played no direct role in spreading the virus.Canada geese were experimentally exposed to turkey/Wisconsin/66 and turkey/Wisconsin/68 viruses; mallard ducks were exposed to turkey/Wisconsin/66 virus. HI antibody developed in 75% of the geese and 40% of the ducks but was generally short-lived. Anti-RNP antibody was detected in 15% of the exposed geese but in none of the ducks. Virus was recovered from 3 of 10 adult ducks but not from geese. None of the birds showed signs of disease.