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Studies on Dictyocaulus filaria. I. Modifications of laboratory procedures.

Authors: Anderson, P.J.S.; Verster, Anna;

Studies on Dictyocaulus filaria. I. Modifications of laboratory procedures.

Abstract

The journals have been scanned in colour with a HP 5590 scanner; 600 dpi. Adobe Acrobat v.11 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. ; Techniques used in a study of the life-cycle of Dictyocaulus filaria (Rudolphi, 1809) are described. First stage larvae migrate from faecal pellets placed in water. Infective larvae are cultured in clean water at room temperature and aerated overnight for 7 days. Sheep are infested per os with infective larvae. At various intervals after infestation they are killed and their organs are cut into small pieces, placed in a modified Baermann apparatus and the parasites allowed to migrate from the tissue into physiological saline at 37°C for 6 to 8 hours. Larvae are collected from lymph drained from the thoracic duct with a catheter. Some of the recovered worms are killed with an iodine solution; others intended for morphological studies are killed by heating to 60°C and preserved in a mixture containing triethanolamine.

Country
South Africa
Related Organizations
Keywords

Sheep, Trichostrongyloidea, Veterinary medicine, Preservation, Biological, Sheep Diseases, Body Fluids, South Africa, Feces, Larva, Veterinary medicine -- South Africa, Dictyocaulus Infections, Methods, Animals

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
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