
pmid: 4277675
handle: 2263/50989
The journals have been scanned in colour with a HP 5590 scanner; 600 dpi. Adobe Acrobat v.11 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. ; Techniques used in a study of the life-cycle of Dictyocaulus filaria (Rudolphi, 1809) are described. First stage larvae migrate from faecal pellets placed in water. Infective larvae are cultured in clean water at room temperature and aerated overnight for 7 days. Sheep are infested per os with infective larvae. At various intervals after infestation they are killed and their organs are cut into small pieces, placed in a modified Baermann apparatus and the parasites allowed to migrate from the tissue into physiological saline at 37°C for 6 to 8 hours. Larvae are collected from lymph drained from the thoracic duct with a catheter. Some of the recovered worms are killed with an iodine solution; others intended for morphological studies are killed by heating to 60°C and preserved in a mixture containing triethanolamine.
Sheep, Trichostrongyloidea, Veterinary medicine, Preservation, Biological, Sheep Diseases, Body Fluids, South Africa, Feces, Larva, Veterinary medicine -- South Africa, Dictyocaulus Infections, Methods, Animals
Sheep, Trichostrongyloidea, Veterinary medicine, Preservation, Biological, Sheep Diseases, Body Fluids, South Africa, Feces, Larva, Veterinary medicine -- South Africa, Dictyocaulus Infections, Methods, Animals
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