Research on the upside-down jellyfish Cassiopea xamachana has increased in the past few decades, hence the need for more efficient husbandry protocols. We tested the effect of weekly feeding frequencies, light cycles, and nutrient supplements on symbiotic and aposymbiotic polyp asexual reproduction and mortality. C. xamachana polyps have better survivorship and reproduction when kept in a day/night cycle and given additional food beyond Artemia nauplii.