Acne vulgaris is an inflammatory skin disease caused by several factors, including an imbalance of microorganisms compared to the normal microbial distribution in healthy tissue. The Lauraceae family which have been reported from antiseptic. This research was conducted to assayed the compounds, the biological activity of the Lauraceae plant essential oil (EO) could against microbes. EO Were isolated by steam water distillation and had antibacterial effects were analyzed by microdilution in broth medium and EO compounds were analyzed by GC-MS. The most abundant components present in N. cassia leaves cinnamyl acetate, N. cassia bark beta-citronellol, linalool, e-citral and geraniol, C. verum bark eugenol, C. burmanni leaves linalool, alpha-terpineol, eucalyptol, cinnamaldehyde, caryophyllene, cinnamyl, leaves of Cinnamomum camphora contains camphor. The highest antimicrobial activity against C. acnes was given by oil from the leaves and bark of Neolitsea cassia, against S. aureus and by oil from the bark of C. verum and N. cassia and C. burmannii leaves. The oil of C. champora leaves gave better activity than the barks against these microbes. It was found that the leaves of C. champora had the highest yield of oil compared to the other part of Lauraceae plants tested and it has a strong antibacterial activity toward microbes commonly present in acne vulgaris.