Ulva lactuca L. contains bioactive substances with anti-inflammatory, antibacterial, and antioxidant properties that aid in healing cut wounds. This research was done to ascertain the histopathological parameter's effects on the wound-healing capacity of gels made from the ethanol extract of U. lactuca (EEUL). The 45 mice were equally divided into five groups: The gel-based control (group I), the positive control (group II), the pain control (group III), and the treatment groups (groups IV and VI), which received EEUL gel at concentrations of 5 and 10%, respectively. On the mice's back, a 1 cm-long incision was made. A one-way ANOVA, post hoc least significant difference (LSD), and/or Mann-Whitney test were used to statistically analyze the data. Reduced wound scores, healing times, and wound length were observed (p 0.05). On the other hand, the macrophage score, the number of blood vessels, the thickness of the epithelium, and the fibroblast count increased (p0.05%). Topical application of 5% or 10% EEUL gel has accelerated wound healing by increasing macrophage scores, blood vessel density, epithelial thickness, and fibroblast counts while decreasing wound description scores and wound length.