
The enzyme-linked immunosorbent assay (ELISA) was used to demonstrate the increase in titer of maize rayado fino virus (MRFV) in its leafhopper vector, Dalbulus maidis. Viral antigen concentration attained a maximum in the body of the insect 25 days after virus acquisition and decreased thereafter. Substantial differences in concentration were observed among viruliferous leafhoppers. MRFV was serially passed through 5 successive leafhopper populations. The results provide further evidence of multiplication of MRFV in D. maidis.
Hemiptera, Animals, Enzyme-Linked Immunosorbent Assay, Antigens, Viral, Zea mays, Plant Viruses
Hemiptera, Animals, Enzyme-Linked Immunosorbent Assay, Antigens, Viral, Zea mays, Plant Viruses
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