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Opal suppressor phosphoseryl-tRNA is not a substrate of phosphoserine aminotransferase.

Authors: T, Mizutani; K, Kanbe; Y, Tachibana; Y, Kimura; T, Hitaka;

Opal suppressor phosphoseryl-tRNA is not a substrate of phosphoserine aminotransferase.

Abstract

A proposal of the role of animal opal suppressor phosphoseryl (Ps)-tRNA is that Ps-tRNA plays a role as an intermediate in the metabolic pathway from 3-phosphoglycerate to glycine. The labeled [32P]phospho[3H]seryl-tRNA was prepared and used as a substrate in the reaction of bovine brain Ps aminotransferase (EC 2.6.1.52) in the presence of alpha-ketoglutarate. By analysis of the reaction product, no amount of 3-phosphohydroxypyruvate was found, even though phosphohydroxypyruvate was noted in the control experiment by use of Ps and alpha-ketoglutarate. These results showed that Ps-tRNA was not a substrate of Ps aminotransferase.

Keywords

Kinetics, Suppression, Genetic, Animals, RNA, Transfer, Amino Acyl, Transaminases, Substrate Specificity

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
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