Embryos of many palm species that produce desiccation-sensitive, i.e. recalcitrant seeds have been successfully cryopreserved. However, storage protocols for some genera such as Borassus are still lacking.Mature fruits of Borassus flabellifer L were collected in south India at the time of natural dispersal (June-July). After removal of the pericarp and mesocarp, the pyrenes were dried in silica gel and the desiccation tolerance level determined. Isolated embryos were also subjected to drying, exposure to liquid nitrogen temperature and recovery in vitro.Mature fruits weighed an average mass of 0.9 kg and germinated to 89%. The moisture contents (MCs) of the pyrenes and embryos were 48 and 78%, respectively. Pyrenes dried in silica gel to c. 20% MC lost viability, whereas embryos could be dried to 11% MC with 55% survival. These results indicate that the seeds are relatively desiccation-sensitive. Embryos dried to 21 and 11% MC had 23 and 36% survival following exposure to liquid nitrogen for 48 h, respectively.There is a hydration level window between c. 10 and 20% MC that is optimal for the cryopreservation of Borassus embryos. However, long-term storage possibilities remain to be explored.