Varroa mite is one of the most dangerous bee parasites and causes a lot of damage to the beekeeping industry. Several chemical and herbal medicines have been used to control this mite so far. In this study, the effects of Artemisia absinthium and Hypericum perforatum prescription to control Varroa mite were investigated. To this end, a total of 380 bee colonies in 5 areas in Kazakhstan were considered. The bitter wormwood herbs and Hypericum extracts were mixed in a ratio of 1:1, and then, prepared in a ratio of 1:10 with sugar syrup (100 ml infusion per 1 liter of sugar syrup). An amount of 200 ml of the prepared solution was used for 5-7 days per colony after medical collection and honey pumping. The percentage of mite infestation at the beginning and end of the experimental period, which was spring, summer, and autumn, and the number of worker mites and worker bees were determined daily. At the beginning of the experiment, the percentage of mite infestation in the experimental hives was not significantly different; however, at the end of the experiment, there was a significant difference between the experimental treatments. The use of a mixture of A. absinthium and H. perforatum extract significantly reduced the abundance index of Varroa mite to 0. Due to the adverse effects of chemical drugs and their high cost, it is recommended to use this extract to control Varroa mite contamination.