The measurement of total serum proteins by the Biuret method.

M M, Lubran

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Article . 1978

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The measurement of total serum proteins by the Biuret method.

descriptionPublicationkeyboard_double_arrow_right Article 08 May 1978 English Journal:Annals of clinical and laboratory science, volume 8 (issn: 0091-7370,

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Authors: M M, Lubran;

pmid: 345944

The measurement of total serum proteins by the Biuret method.

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Abstract

The biuret reaction for proteins provides a simple and precise method for measuring serum proteins; Beer's law is obeyed to at least 10 g per dl. Several stable biuret reagents are available. Hemoglobin is the only important cause of interference which cannot be minimized by use of a sample blank. The mechanism of the biuret reaction is described and attention is drawn to the heterogeneity of the serum proteins and to the use of a certified albumin standard.

Keywords

Biuret Reaction, History, 19th Century, Blood Proteins, Chemistry, Analytic, History, 20th Century

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