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The Journal of General and Applied Microbiology
Article . 2021 . Peer-reviewed
Full-Text: https://www.jstage.jst.go.jp/article/jgam/67/3/67_2020.09.001/_pdf
Data sources: Japan Link Center
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The Journal of General and Applied Microbiology
Article . 2021
Data sources: Europe PubMed Central
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Construction of transcription factor gene deletion library of Aspergillus luchuensis.

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2021 English Publisher:Applied Microbiology, Molecular and Cellular Biosciences Research FoundationJournal:The Journal of general and applied microbiology, volume 67 (issn: 1349-8037, Copyright policy )
Authors: Yamada, Osamu; Nishibori, Naoko; Hayashi, Risa; Arima, Toshihide; Mizutani, Osamu;

pmid: 33883333

Construction of transcription factor gene deletion library of Aspergillus luchuensis.

Abstract

We selected 96 genes of Aspergillus luchuensis for the construction of a transcription factor gene deletion library. Of these, we successfully deleted 93 genes using Agrobacterium-mediated transformation (AMT) of A. luchuensis RIB 2604 ΔligD strains. We obtained only heterokaryonic strains after deletions of adaB, anBH1, hacA, hapB, hsf1, metR, and sonC gene, and additionally, could not obtain deletion strains for genes abaA and mcmA. The deletion strains will be available through our website (https://www.nrib.go.jp).

Related Organizations
Keywords

<i>Aspergillus luchuensis</i>, Aspergillus, Transformation, Genetic, Agrobacterium, deletion library, transcription factor, Gene Deletion, Gene Library, Transcription Factors

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
5
Top 10%
Average
Top 10%
gold