Cellular senescence has been now shown to be at the root of many degenerative processes that characterize ageing. Thus, in addition to an irreversible proliferative arrest following various stresses or inappropriate stimuli, cellular senescence leads to genetic, epigenetic, metabolic, structural and functional adaptations of the cell. Moreover, senescence phenotype spreads to the surrounding tissue through a specific secretory profile. Eliminating or blocking the action of senescent cells by senotherapeutic agents prevents tissue degeneration and improves the longevity in preclinical models. In this review, we will present the latest applications in senotherapy and discuss the very promising results of the first recently published clinical trials.