Neuraminidase fluorescence test for the differentiation of Trypanosoma cruzi and Trypanosoma rangeli.

J, Schottelius

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Article . 1988

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Neuraminidase fluorescence test for the differentiation of Trypanosoma cruzi and Trypanosoma rangeli.

descriptionPublicationkeyboard_double_arrow_right Article 11 Apr 1988 English Journal:Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ), volume 38 (issn: 0177-2392,

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Authors: J, Schottelius;

pmid: 3327143

Neuraminidase fluorescence test for the differentiation of Trypanosoma cruzi and Trypanosoma rangeli.

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Abstract

The cell free supernatants of 13 stocks of Trypanosoma rangeli/T. rangeli-like, 50 stocks of T. cruzi/T. cruzi-like and 3 stocks of T. conorhini grown in culture were examined by using the 2-(4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid) test. It could be demonstrated that only the supernatants of the T. rangeli/T. rangeli-like stocks showed a white-yellow fluorescence under UV light. In contrast, the cell free supernatants of the T. cruzi/T. cruzi-like and T. conorhini stocks showed an ash-grey colour under UV light undistinguishable from that of the control reactions. The 4-MUAc test could be a method of choice for the routine detection of T. rangeli/T. rangeli-like flagellates in culture.

Related Organizations

Bernhard Nocht Institute for Tropical Medicine
Germany
Leibniz Association
Germany

Keywords

Trypanosoma, Spectrometry, Fluorescence, Ultraviolet Rays, Trypanosoma cruzi, Animals, Humans, Neuraminidase

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