With gradually in-depth understanding of wound healing mechanism, the concept and composition of wound microenvironment have been improved and clarified. In the process of wound healing, the external microenvironment and internal microenvironment are both relatively independent and interact with each other, and are always in dynamic change. It is necessary to understand the changing rules and characteristics of wound healing microenvironment from multiple dimensions, such as space and time, so that the process of wound healing can be purposefully regulated and controlled depending on actual circumstance. It helps the adoption of reasonable regenerative medicine technology at the proper time window to realize perfect repair and regeneration after skin and soft tissue injury in the true sense.