[Genetic study of plasmid integration into yeast chromosomes. V. Mapping of integration sites for the plasmid pYF91].

S A, Bulat; E V, Mezhevaia; V P, Stepanova; B F, Iarovoĭ; I A, Zakharov

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Генетика

Article . 1987

Data sources: Europe PubMed Central

[Genetic study of plasmid integration into yeast chromosomes. V. Mapping of integration sites for the plasmid pYF91].

descriptionPublicationkeyboard_double_arrow_right Article 10 Dec 1987 Russian Journal:Genetika, volume 23 (issn: 0016-6758,

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Authors: S A, Bulat; E V, Mezhevaia; V P, Stepanova; B F, Iarovoĭ; I A, Zakharov;

pmid: 3311880

[Genetic study of plasmid integration into yeast chromosomes. V. Mapping of integration sites for the plasmid pYF91].

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Abstract

The data on mapping the episomal plasmid integration sites in yeast chromosomes I, III, IV, V, VII, XV are presented. In addition to the integration site at leu2 of chromosome III localized earlier, 6 more loci containing apparently the homologous yeast transposons, with a copy in a plasmid, were defined. The fact of plasmid integration was proved by colony hybridization technique with the pBR322 probe. The plasmid DNA segregation (the ratio 2:2) and its linkage to pLEU2 plasmid marker gene were observed in hybrids of all integrants studied.

Keywords

Genetic Markers, Recombination, Genetic, Chromosome Mapping, Saccharomyces cerevisiae, DNA, Fungal, Plasmids

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