In recent years, male infertility has received more and more attention from scholars at home and abroad. Asthenospermia is one of the common causes of male infertility, the main manifestation of which is low sperm motility. Current studies show that the pathogenic factors for asthenospermia are complex and diverse, including gene mutations, alteration of ion channels, oxidative stress, changes in the contents of trace elements, and others. Despite the progress made in the related researches, the mechanisms underlying the pathogenesis of asthenospermia have not yet been fully elucidated. With a review of the recent relevant literature published at home and abroad, this article presents an overview on the related gene mutations, alteration of ion channels, changes in the levels of proteins, epigenetics, and other molecular biological mechanisms underlying the pathogenesis of asthenospermia, hoping to provide some evidence for the clinical diagnosis and treatment of asthenospermia.