Objective:To report surgical techniques, outcomes, clinical experience and application key point of transcanal endoscopic ear surgery for tympanic antrum cholesteatoma. Method:Data from 27patients who underwent transcanal endoscopic ear surgery for tympanic antrum cholesteatoma were retrospectively analyzed. Removal of tympanic antrum cholesteatoma and tympanoplasty were individualized. Patients were followed up at postoperative 1, 3 and 6month. Result:There was no failure of tympanal and canal wall reconstruction. The mean preoperative air conduction threshold was（50.33±7.34） dB HL, compared to（29.59±5.56） dB HL postoperatively P=0.00）. The mean preoperative air-bone gap was（29.13±4.70） dB, compared to（12.25±4.98） dB postoperatively（P=0.00）. There was no complication, recurrence, residual tympanic antrum cholesteatoma or secondary surgery needed. Conclusion:Stand alone transcanal endoscopic ear surgery is a safe and effective for tympanic antrum cholesteatoma.