In the JSCC, recommended methods for eight items of enzymes and 9 items of non-enzyme (one item for Na, K, and Cl) have been presented since the recommended methods of activity measurements of AST, ALT, CK, and LD were prepared in 1989. These recommended methods were prepared by placing priority on the actual situation of the measurement methods of those days in Japan, and there are some differences with IFCC recommended methods or primary reference procedures. Recently, from the viewpoint of global standardization, measured values that can be shared with data overseas are required even on clinical exami- nation, and overseas inspection facilities are sometimes used to avoid differences in measurement methods in international clinical trials. With this situation, when the recommended methods of JSCC and IFCC are compared, there are the following differences: - PALP for AST and ALT: not added in JSCC method/ added in IFCC method. - Buffer solution (pH) for LD: EAE (pH 8.8) in JSCC method/ NMG (pH 9.4) in IFCC method. Buffer solution for ALP: EAE in JSCC method/ AMP in IFCC method. Free glycerol for TG: not included on JSCC method/ included in ID-GC/MS method of NIST and CDC. It is necessary to consider from various aspects whether or not these differences affect the implementation of global standardization. In consideration, it is necessary to add the point of how the method should be linked directly to the international traceability of CRMLN (Cholesterol Reference Method Laboratory Net- work) and JCTLM (Joint Committee for Traceability in Laboratory Medicine) to the domestic surveillance and to judge it. [Review].