Objective To observe the prevention of acupoint massage for the occurrence of deep venous thrombosis (DVT) of various risk degrees after gynecologic laparoscopic surgery. Methods Using Autar deep vein risk assessment scale, patients undergoing gynecological laparoscopic surgery from June 2014 to December 2014 were assessed one day before surgery and 24 h after surgery. Patients at moderate and high risks were recruited as subjects in this research by blocking method, 72 in the moderate risk group and 34 in the high risk group. Patients in the two groups were then randomly assigned to the control group and the test group respectively. Conventional care plus intermittent limb pressure treatment were performed to patients in the control group. Conventional care plus acupoint massage [Zusanli (ST36) , Shangjuxu (ST37) , Liangqiu (ST34) , Yinshi (ST33) , Futo (ST32) ] were performed to patients in the test group. Red blood cell ( RBC) aggregation index and popliteal vein blood flow velocity were detected before surgery, at day 1 and 5 after surgery. The postoperative thrombosis rate was statistically analyzed. Results DVT of lower limbs occurred in 4 cases of this study, 2 in the moderate risk group and 2 in the high risk group. The incidence of DVT of lower limbs was 5. 6% (2R6) in the moderate risk control group and 0. 0% (0/36) in the moderate risk test group, and it was 11. 8%(2/17) in the high risk control group and 0. 0% (0/17) in the high risk test group (P <0. 05). The incidence of thrombosis in recruited patients was significantly lower in the test group than in the control group (P <0. 05). Compared with before surgery, RBC aggregation index obviously increased and popliteal vein blood flow velocity significantly decreased in recruited patients at day 1 after surgery (P <0. 05). Compared with day 1 after surgery, RBC aggregation index obviously increased and popliteal vein blood flow velocity significantly decreased in all patients at day 5 after surgery (P <0. 05). Compared with the control group, RBC aggregation index obviously decreased and popliteal vein blood flow velocity significantly increased in the test group (P <0. 05). Conclusion As for moderate risk and high risk patients evaluated by Autar scale, conventional care and assisted acupoint massage was conducive to preventing postoperative DVT.