
In this work the complete sequence of 1097 nucleotide bovine beta-casein cDNA has been determined. Sequencing of end labeled fragments was performed using the method of Maxam and Gilbert. Bovine beta-casein cDNA consist of a 672 nucleotide coding region, flanked by 60 nucleotide 5' and 358 nucleotide 3'-noncoding region. The restriction map of beta-casein cDNA was constructed. The computer analysis was done and the comparisons of the complete sequence of bovine beta-casein cDNA with other sequences, coding caseins in bovine, rat and guinea-pig was performed. It was determined, that cloned cDNA is related to genetic variant A1.
Base Sequence, Guinea Pigs, Caseins, DNA, DNA Restriction Enzymes, Rats, Genes, Species Specificity, Sequence Homology, Nucleic Acid, Animals, Cattle, Amino Acid Sequence, Cloning, Molecular
Base Sequence, Guinea Pigs, Caseins, DNA, DNA Restriction Enzymes, Rats, Genes, Species Specificity, Sequence Homology, Nucleic Acid, Animals, Cattle, Amino Acid Sequence, Cloning, Molecular
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