Objective:To report surgical techniques, outcomes, clinical experience and application key point of transcanal endoscopic ear surgery for tympanosclerosis. Method: Data from 25 patients (13 males and 12 females; aged 27-61 years, mean=48.8 years) who underwent transcanal endoscopic ear surgery for tympanosclerosis in our department from January 2016 to June 2017 were retrospectively analyzed. Removal of tympanosclerosis and tympanoplasty were individualized. Patients were followed up at postoperative 1, 3 and 6 month. Result: There were no failure of tympanal reconstruction by sandwich. The mean preoperative air conduction threshold was higher than that of postoperation ［(51.12±16.03）dB HL and （31.64±12.76)dB HL, respectively］(P<0.01). The mean preoperative air-bone gap was significantly increased compared to that of postoperation ［(30.33±10.12）dB and （14.19±8.97）dB,respectively］ (P<0.01). There were no complication, recurrence, residual tympanosclerosis or secondary surgery needed. Conclusion: Stand alone transcanal endoscopic ear surgery was a safe and effective method for tympanosclerosis.