[Expression of DKKL1 in spermatozoa of men with asthenospermia].
Copyright policy )[Expression of DKKL1 in spermatozoa of men with asthenospermia].
To compare the expression of DKKL1 in ejaculated spermatozoa of normal fertile men and men with asthenospermia and investigate the role of DKKL1 in the pathogenesis of asthenospermia.The characteristics of semen samples collected from normal fertile men and men with asthenospermia were analyzed using computer-assisted sperm analysis according to WHO criteria. The ejaculated sperms were isolated by Percoll discontinuous density gradients to detect the expression of DKKL1 mRNA and protein using real-time PCR and Western blotting.The expression of DKKL1 mRNA was significantly down-regulated by 11.1 times in asthenospermic men as compared with that in normal fertile men (P<0.01). Western blotting showed that the expression of DKKL1 protein was down-regulated by 2.4 times in asthenospermic men compared to normal fertile men.The expression of DKKL1, which may play an important role in sperm motility,is significantly decreased in ejaculated spermatozoa of men with asthenospermia.
- Guangzhou Medical University China (People's Republic of)
- Jinan University China (People's Republic of)
Male, Asthenozoospermia, Case-Control Studies, Blotting, Western, Sperm Motility, Humans, Intercellular Signaling Peptides and Proteins, RNA, Messenger, Spermatozoa
Male, Asthenozoospermia, Case-Control Studies, Blotting, Western, Sperm Motility, Humans, Intercellular Signaling Peptides and Proteins, RNA, Messenger, Spermatozoa
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).1 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!