[Properties of DNA-(cytosine-5-)-methyltransferase in the brain].

A, Pfohl-Leszkowicz; G, Keith; G, Dirheimer

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Article . 1986

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[Properties of DNA-(cytosine-5-)-methyltransferase in the brain].

descriptionPublicationkeyboard_double_arrow_right Article 30 May 1986 French Journal:Comptes rendus des seances de la Societe de biologie et de ses filiales, volume 179 (issn: 0037-9026,

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Authors: A, Pfohl-Leszkowicz; G, Keith; G, Dirheimer;

pmid: 2938697

[Properties of DNA-(cytosine-5-)-methyltransferase in the brain].

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Abstract

Beef brain DNA-(cytosine-5-)-methyltransferase was partially purified by chromatography on Ultrogel AcA34 and Dyematrex Blue A. The purification was of 360 times and the recovery of 75%. The pH optimum of the reaction is 7.6 NaCl inhibits double stranded DNA methylation, but stimulates single stranded DNA methylation up to 50 mM, before inhibiting. EDTA (1 mM) and MgCl2 (4 mM) stimulate DNA methylation. Polyamines inhibit the reaction.

Keywords

Kinetics, Cations, Divalent, Chromatography, Gel, Animals, Brain, Cattle, DNA (Cytosine-5-)-Methyltransferases, Hydrogen-Ion Concentration, Chromatography, Ion Exchange, Edetic Acid

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