Direct interaction between VRN1 protein and the promoter region of the wheat FT gene.
Copyright policy )Direct interaction between VRN1 protein and the promoter region of the wheat FT gene.
The wheat florigen gene Wheat FLOWERING LOCUS T (WFT, which is identical to VRN3) is an integrator of the vernalization, photoperiod and autonomous pathways in wheat flowering. Many studies have indicated that VERNALIZATION 1 (VRN1) directly or indirectly up-regulates WFT expression in leaves. VRN1 encodes an APETALA1/FRUITFULL-like MADS box transcription factor that is up-regulated by vernalization and aging, leading to promotion of flowering. In this study, the VRN1 protein was expressed as a His-Tag fusion protein in Escherichia coli and used in an electrophoretic mobility shift assay (EMSA). The results from the EMSA indicated that the VRN1 protein directly binds to the CArG-box in the promoter region of WFT, suggesting the direct up-regulation of WFT by VRN1 in the leaves of wheat plants.
- Kyoto University Japan
- Fukui Prefectural University Japan
flowering, CArG-box, MADS Domain Proteins, Up-Regulation, Plant Leaves, Gene Expression Regulation, Plant, wheat (<i>Triticum aestivum</i>), Cloning, Molecular, <i>WFT</i>, Promoter Regions, Genetic, VRN1 protein, Triticum, Plant Proteins, Protein Binding
flowering, CArG-box, MADS Domain Proteins, Up-Regulation, Plant Leaves, Gene Expression Regulation, Plant, wheat (<i>Triticum aestivum</i>), Cloning, Molecular, <i>WFT</i>, Promoter Regions, Genetic, VRN1 protein, Triticum, Plant Proteins, Protein Binding
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