Bordetella pertussis filamentous hemagglutinin gene: molecular cloning of a potential coding sequence.
Copyright policy )Bordetella pertussis filamentous hemagglutinin gene: molecular cloning of a potential coding sequence.
We have constructed an expression library of Bordetella pertussis DNA sequences, cloned in Escherichia coli. Random DNA fragments were inserted into the beta-galactosidase gene of bacteriophage lambda gt11 and plaques were screened with antibodies directed against filamentous hemagglutinin. The antigen-positive clone obtained expressed B. pertussis sequences as polypeptides fused to beta-galactosidase. The fusion polypeptides reacted both with antibodies to beta-galactosidase and with antibodies to filamentous hemagglutinin. The positive clone contains B. pertussis DNA sequences as determined by specific hybridization to Bordetella DNA.
DNA, Bacterial, Hemagglutinins, Base Sequence, Genes, Bacterial, Nucleic Acid Hybridization, Cloning, Molecular, Bordetella pertussis
DNA, Bacterial, Hemagglutinins, Base Sequence, Genes, Bacterial, Nucleic Acid Hybridization, Cloning, Molecular, Bordetella pertussis
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).0 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!