Identification of sex from skeletal remains is an important tool in forensic science. Mandibular ramus can be used for sex determination either on dry mandible or through orthopantomogram (OPG).To determine the sex from mandibular ramus using digital OPG.The morphometric analysis was conducted on mandibular ramus of 1000 digital OPG using Kodak Master View version 4.3 software. Statistical analysis was performed, and independent t-test and discriminant function were applied.The participants' age ranged from 21-60 years with an equal number of males and females. The mean dimensions of all parameters for ramus were higher in males and highly significant (P < 0.001). The total mean length of minimum and maximum ramus breadth was 27.44 ± 3.41 mm and 32.27 ± 3.40 mm, respectively. The maximum and projective ramus height was 71.78 ± 5.98 mm and 65.62 ± 6.19 mm, respectively. The coronoid height was 59.23 ± 6.08 mm. The correlation of gender with morphology of mandibular ramus was significant (P < 0.05). The overall accuracy for diagnosing sex was 69%, whereas for diagnosing male and female, the accuracy was 68% and 70%, respectively.Measurements of mandibular ramus using OPG are helpful in sex determination.