Dot-enzyme-linked immunosorbent assay (dot-ELISA) for the diagnosis of schistosomiasis.

H A, Hamadto; K A, Kamal

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Journal of the Egypt...arrow_drop_down

Journal of the Egyptian Society of Parasitology

Article . 1989

Data sources: Europe PubMed Central

Dot-enzyme-linked immunosorbent assay (dot-ELISA) for the diagnosis of schistosomiasis.

descriptionPublicationkeyboard_double_arrow_right Article 09 Nov 1989 English Journal:Journal of the Egyptian Society of Parasitology, volume 19 (issn: 1110-0583,

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Authors: H A, Hamadto; K A, Kamal;

pmid: 2794579

Dot-enzyme-linked immunosorbent assay (dot-ELISA) for the diagnosis of schistosomiasis.

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Abstract

We describe a dot enzyme-linked immunosorbent assay (dot-ELISA) as a field applicable tool for the rapid diagnosis of schistosomiasis. This antibody capture assay is performed using 50 ng protein of crude Schistosoma mansoni egg antigen (SEA) in 1 microliter volumes per dot. Sera (1 microliter/dot) at 1:40 dilution were optimal. Dot-ELISA results were completely comparable to micro-ELISA.

Keywords

Male, Adolescent, Immunoblotting, Antibodies, Helminth, Animals, Humans, Schistosoma, Schistosomiasis, Enzyme-Linked Immunosorbent Assay, Child

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