[Enzyme immunoassay for thromboxane B2].
Copyright policy )[Enzyme immunoassay for thromboxane B2].
A sensitive and specific enzyme immunoassay for TXB2, the stable degradation product of thromboxane A2, was developed, in which the hapten molecule was labeled with pure peroxidase. After competitive binding to antibody between enzyme-labeled and free TXB2, the immunoreactive product was precipitated by double antibody technique, and the enzyme activity of the precipitate was determined spectrophotometrically. The procedures allowed a determination of 3-200 pg TXB2/tube (0.081 to 5.4 nmol/l).
Immunoenzyme Techniques, Thromboxane B2, Predictive Value of Tests, Spectrophotometry, Guinea Pigs, Humans, Animals, Reference Standards, Binding, Competitive
Immunoenzyme Techniques, Thromboxane B2, Predictive Value of Tests, Spectrophotometry, Guinea Pigs, Humans, Animals, Reference Standards, Binding, Competitive
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