The aim of this study was to determine the influence of two different glide path preparation methods on the fracture rate of the Primary 25/08 WaveOne reciprocating instrument. Preparation times for different glide path methods and total time for root canal preparation, with and without prior glide path preparation, were also calculated.ISO 15, 0.02 taper Endo-Training-Blocks (n = 300) were selected and randomly divided into three main groups (n = 100): Group 1: no glide path (control); Group 2: glide path preparation with stainless-steel hand files; and Group 3: glide path preparation with rotary PathFiles. The time taken to prepare each glide path was recorded. The specimens in each main group were then randomly assigned into five subgroups (n=20). New Primary 25/08 WaveOne files were used for canal preparation in each subgroup. The outcome was measured by recording how many simulated canals could be shaped with one WaveOne reciprocating file in each subgroup before instrument breakage occurred. The average time it took to prepare each of the simulated canals was calculated and recorded. The data were collected and statistically analysed using the ANOVA / Bonferroni test.Glide path preparation with PathFiles was significantly faster than with hand files (P<0.001). After the glide path preparation had been performed with PathFiles, a greater number of simulated canals could be prepared before failure of the WaveOne file (P<0.001). Root canal preparation time was significantly shorter (P<0.001) when an initial glide path had been prepared with PathFiles.