The parasitic phase of Anocentor nitens (Neumann) in cattle was studied. Larval stage was present up to 15 days post-infestation (p.i.), concomitant with nymphae from day 8 p.i. Nymphae were observed up to day 23 p.i., adults being seen from day 15 p.i. onwards. Male ticks disclosed active movements from day 17 p.i. and detachment of engorged females began 22 to 24 days p.i. Massive detachment occurred from day 28 p.i. Preferential sites of parasitic stages were ear and neck (lateral side).