Autopsy represents traditional and classical examination in forensic medicine. It was suggested that conventional autopsy, nowadays sometimes rejected or not tolerated by people for its damage to corpse. Virtopsy offers a non-invasive investigation approach and can also reflect fracture, soft-tissue injury, wound track, organ trauma objectively and accurately. Thus, virtopsy can provide visual and powerful evidence for forensic pathological practice. This article mainly synthesizes the latest literature at home and abroad and reviews application of virtopsy in forensic trauma identification, mechanical asphyxia, drowning, hypothermia and hyperpothermia, disease diagnosis and research of finite element method applicated in trauma investigation to illustrate this new direction of forensic research.