45Ca uptake by retinal pigment epithelial cells.
Copyright policy )45Ca uptake by retinal pigment epithelial cells.
Uptake of 45Ca was studied in isolated frog retinal pigment epithelial cells. 45Ca accumulation was found to be a saturable, temperature-dependent event. Kinetic analysis of this accumulation revealed two transport systems with apparent km of 2.0 and 0.3 mM. We found the presence of a Na-Ca exchanger mechanism that releases Ca2 under depolarized conditions. Light induced an increase of 45Ca uptake due to activation of the Na-K-ATPase and consequent decrease of extracellular potassium concentration.
Light, Calcium Radioisotopes, Osmolar Concentration, Sodium, Kinetics, Animals, Calcium, Sodium-Potassium-Exchanging ATPase, Ouabain, Pigment Epithelium of Eye, Potassium Cyanide
Light, Calcium Radioisotopes, Osmolar Concentration, Sodium, Kinetics, Animals, Calcium, Sodium-Potassium-Exchanging ATPase, Ouabain, Pigment Epithelium of Eye, Potassium Cyanide
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