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[Construction of libraries of structural genes (cDNA) and "jumping" gene libraries in lambda vectors].

Authors: E R, Zabarovskiĭ; R L, Allikmets;

[Construction of libraries of structural genes (cDNA) and "jumping" gene libraries in lambda vectors].

Abstract

Main approaches and methods used for constructing "jamping" and cDNA libraries, including novel ones, that omit the employment of methylases and linkers, are presented. The advantages and drawbacks of the well-known and new lambda vectors, suitable for the purposes mentioned, are discussed. Special attention is paid to diphasmids lambda ZAP, lambda SK12, lambda SK15, that combine features of lambda and M13 phages and of plasmids. The convenience and difficulties of "jumping" libraries for physical mapping of chromosomes are briefly considered.

Keywords

Base Sequence, Genes, Genetic Vectors, Molecular Sequence Data, DNA, Bacteriophage lambda, Gene Library

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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Average
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