The purpose of this study was to compare school and non-school dental laboratories in marginal adaptation of single Porcelain-Fused-to-Metal (PFM) crowns.Six dental laboratories (three school- and three non-school laboratories) participated in this study. Each dental laboratory was requested to fabricate PFM crowns for three different ivorine teeth on different typodont jaw models (n=18). The crowns received from the laboratories and fitted in their abutments using fit-checker under uniform pressure. We examined 162 sites viewed under a scanning electron microscope to measure the vertical and horizontal marginal discrepancy.The mean vertical marginal discrepancy for school dental laboratories was 208.9 µm, while for non-school dental laboratories was 182.8 µm. For the horizontal marginal discrepancy, the median values were 48.5 µm for school dental laboratories and 50 µm for non-school dental laboratories.There was no statistically significant difference in the mean vertical and median horizontal values between school and non-school dental laboratories. However, a significant difference was observed within the non-school laboratories.