To establish an HPLC fingerprint of Eriobotrya japonica flower.13 batches of Eriobotrya japonica flowers from different habitats were analyzed by HPLC with ursolic acid as reference substance. The chromatographic condition was performed on Alltech Apollo C18 column (250 mm x 4.6 mm, 5 microm), eluted with mobile phase containing methanol and 0.1% glacial acetic acid in gradient mode. The flow rate was 0.5 mL/min and the detection wavelength was set at 210 nm. The temperature of column was 20 degrees C.The HPLC fingerprint of Eriobotrya japonica flower had been established. There were 17 common peaks, two of which were identified by reference substances. 13 batches of sample from different habitats can be distinguished from their fingerprints.This method is reasonable and reliable, which can be used for quality evaluation of Eriobotrya japonica flower.