To establish the HPLC fingerprint of ethanol extract of Phellinus igniarius ( EEPI), and to evaluate its quality.Adopted 0.2% phosphoric acid -methanol as mobile phase with a SHISEIDO CAPCELL PAK C18 column (250 mm x 4.6 mm, 5 microm), the flow rate was 1.0 mL/min,and the detection wavelength was set at 395 nm. Inoscavin A was used as reference peak to establish common mode of characteristic absorption peaks of EEPI, and its similarity was evaluated by peaks overlap rate calculation,similarity evaluation system and cluster analysis.Under the chromatographic conditions, HPLC fingerprint of EEPI was established, and nine common peaks were selected. The similarities was between 0.652 and 0.995. According to cluster analysis, 10 batches of Phellinus igniarius were divided into three groups.This method is simple, accurate and repeatable with good separation. It can provide basis for the comprehensive quality evaluation of Phellinus igniarius.