To study the HPLC fingerprint of immunosuppressive fraction of Rehmanniae Radix, the genuine medicinal materials in Henan province.By HPLC method,Dikma Platisil-C18 column (250 mm x 4.6 mm, 5 microm) was used with acetonitrile-0.1% phosphoric acid as mobile phase by gradient elution. The detection wavelength was 210 nm, flow rate was 1.0 mL/min and column temperature was set at 30 degrees C.Compared the 10 batches sample of the fingerprint similarity, 18 common peaks were marked and five peaks were identified. The similarities were more than 0.9.HPLC fingerprint of immunosuppressive fraction of Rehmanniae Radix is established, which lays the foundation for further pharmacodynamics research on Rehmanniae Radix.