Human sphingolipid activator protein-1 and sphingolipid activator protein-2 are encoded by the same gene.
Copyright policy )Human sphingolipid activator protein-1 and sphingolipid activator protein-2 are encoded by the same gene.
Mixed oligonucleotide primers complementary to the translation product of the sphingolipid activator protein (SAP)-2 were used to generate a 144-base pair (bp) complementary DNA (cDNA). This cDNA probe was used to isolate a 2,649-nucleotide-long cDNA that was sequenced and found to contain coding sequences for two known activators of lysosomal enzymes, namely, the sphingolipid activator protein (SAP)-1 and SAP-2. The cDNA contains an open reading frame of 1,482 nucleotides and 1,167 nucleotides of 3'-nontranslated region, followed by a stretch of 24 residues of adenylic acid. At 20 nucleotides upstream from the poly(A) tail there is a consensus AATAAA polyadenylation signal that is preceded by another potential polyadenylation signal. The cDNA, designed SAP-1/SAP-2 cDNA, hybridizes with two human mRNA species of approximately 3 kb in length, which most probably arise from polyadenylation at different sites. There are higher amounts of steady-state RNA levels of the SAP-1/SAP-2 mRNA in skin fibroblasts in comparison to B cells. The steady-state SAP-1/SAP-2 mRNA levels in Gaucher B cells are higher than in their normal counterparts. There is one human SAP-1/SAP-2 gene that has been cloned and is localized on two approximately 5 kb BamHI fragments.
Sphingolipid Activator Proteins, Base Sequence, Placenta, Molecular Sequence Data, Restriction Mapping, DNA, Blotting, Northern, Polymerase Chain Reaction, Saposins, Cell Line, Genes, Pregnancy, Sequence Homology, Nucleic Acid, Humans, RNA, Female, Amino Acid Sequence, Oligonucleotide Probes, Gene Library, Glycoproteins
Sphingolipid Activator Proteins, Base Sequence, Placenta, Molecular Sequence Data, Restriction Mapping, DNA, Blotting, Northern, Polymerase Chain Reaction, Saposins, Cell Line, Genes, Pregnancy, Sequence Homology, Nucleic Acid, Humans, RNA, Female, Amino Acid Sequence, Oligonucleotide Probes, Gene Library, Glycoproteins
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).24 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!