The main goal of this research was to determine the frequency of hantavirus-specific antibodies in rodents from the municipality of San Marcos in the Sucre department of Colombia.144 rodents were captured in San Marcos' urban and rural areas using Sherman traps between December 2007 and July 2009. "Virus sin Nombre" (SNV)-specific antibodies were detected in plasma samples by an indirect ELISA immunoassay.An 8.3 % (12/144) seroprevalence rate was found. Specific seropositivity rates ranged from 6.8 % (3/44, Zygodontomysbrevicauda) to 50 % (1/2, Neacomysspinosus). No significant differences were found in seroprevalence according to capture area, gender and/or reproductive stage (p>0.05); however, there were more seropositive adult males.This is the first evidence of hanta virus seropositivity in rodents from the Murinae subfamily in Colombia. The presence of SNV antibodies in rodents in San Marcos supported the hypothesis that at least one hantavirusis circulating in rodents from northern Colombia.