Poly(A) polymerase-based poly(A) length assay.

Deepak P, Patil; Baskar, Bakthavachalu; Daniel R, Schoenberg

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Article . 2014

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Poly(A) polymerase-based poly(A) length assay.

descriptionPublicationkeyboard_double_arrow_right Article 04 Nov 2014 English Journal:Methods in molecular biology (Clifton, N.J.), volume 1,125 (issn: 1940-6029,

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Authors: Deepak P, Patil; Baskar, Bakthavachalu; Daniel R, Schoenberg;

pmid: 24590776

pmc: PMC3951053

Poly(A) polymerase-based poly(A) length assay.

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Abstract

mRNA polyadenylation functions in nuclear export, translation, and stability. We describe an efficient protocol designed to assess poly(A) tail length that is based on 3' tailing by yeast poly(A) polymerase and product analysis to single-nucleotide resolution by capillary electrophoresis.

Related Organizations

The Ohio State University
United States

Keywords

Genetic Techniques, Electrophoresis, Capillary, Polynucleotide Adenylyltransferase, RNA, Messenger, Poly A

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